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Lesson Plan: Cloning DNA Sequences Biology • Third Year of Secondary School
This lesson plan includes the objectives, prerequisites, and exclusions of the lesson teaching students how to describe the process of creating recombinant DNA using vectors and outline how reverse transcriptase can be used to clone sections of DNA.
Objectives
Students will be able to
- define the term recombinant DNA as DNA that has been taken from two sources and combined,
- explain how plasmids can be used as vectors for inserting pieces of DNA into and cloning,
- describe the roles of restriction enzymes and DNA ligase in cloning pieces of DNA,
- describe how bacterial cells can be transformed and used to make multiple clones of a piece of DNA,
- suggest how reverse transcriptase can be used to obtain a sequence of DNA,
- recall that PCR can be used to amplify sections of cloned DNA.
Prerequisites
Students should already be familiar with
- the fact that DNA is the genetic material of the cell.
Exclusions
Students will not cover
- the details of the PCR process.
