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Question Video: Identifying the Characteristics of an Enzyme Biology • Second Year of Secondary School

Which of the following is not a characteristic of enzymes? [A] They are affected by pH and temperature. [B] They are specific in their action. [C] They speed up the rate of reaction. [D] They cannot be reused after the reaction is complete.

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Video Transcript

Which of the following is not a characteristic of enzymes? (A) They are affected by pH and temperature. (B) They are specific in their action. (C) They speed up the rate of reaction. Or (D) they cannot be reused after the reaction is complete.

A reaction is a chemical process in which substances called reactants are transformed into different substances called products. For a reaction to proceed, a certain amount of activation energy is needed. Let’s look at an example.

Starch is a relatively stable macromolecule, which is found in a lot of the food we eat. When we eat food containing starch, our body cannot take the starch up as it is. This is because it is too large. So, in order for our body to take up starch molecules, they need to be broken down into smaller sugar molecules. This happens during digestion. For the reaction which breaks down starch into smaller molecules to happen by itself, without the help of enzymes, lots of energy is required. Enzymes speed up such reactions by lowering the energy needed for the change of conformation of molecules.

The question asks about the characteristics of enzymes, so let’s repeat them. We have already repeated that enzymes speed up the rate of reaction. As the question asks for the answer choice which does not describe a characteristic of enzymes, we can exclude answer option (C).

Enzymes are highly specific to the substrate which they help to transform into a product. In our starch example, the enzyme amylase has an active site which is highly specific to bind to starch molecules. Starch is therefore the substrate of amylase. When the starch molecule is bound to the enzyme in an enzyme–substrate complex, the enzyme helps to break bonds within the starch. At the end of this process, amylase releases maltose molecules as products, which are more simple sugars. So answer choice (B) is incorrect, as a characteristic of enzymes is that they are specific in their action.

After having freed the product out of its active site at the end of the reaction, the enzyme is ready to bind to the next substrate to perform its action again. So answer choice (D) seems to be our correct answer, as enzymes can be reused after the reaction is complete. But let’s discuss the last answer choice just to be sure.

Enzymes are indeed affected by pH and temperature. Every enzyme has a specific pH range in which it works best. Our mouth has quite a neutral pH. That’s where salivary amylase breaking down starch into maltose is found and works best. In a more or less acidic environment, it denatures and cannot work anymore. In terms of temperature, most enzymes in our body have a similar preference. This is because our general body temperature is at around 37 degrees Celsius and our enzymes are adapted to this. If they’re in a colder environment, they are less active. If the temperature exceeds a certain rate, the enzymes degenerate and cannot work anymore.

Now we can answer with certainty that the statement which is not describing a characteristic of enzymes is statement (D): they cannot be reused after the reaction is complete.

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