Worksheet: Column Chromatography

In this worksheet, we will practice describing purification by column chromatography, selecting mobile and stationary phases and troubleshooting errors.

Q1:

Which of the following statements about columns containing more theoretical plates is true?

  • AColumns containing more theoretical plates interact irreversibly with the analyte.
  • BColumns containing more theoretical plates take a long time to perform separations.
  • CColumns containing more theoretical plates lend themselves to component mixing.
  • DColumns containing more theoretical plates are better suited to separate a complex mixture.
  • EColumns containing more theoretical plates make separations impossible.

Q2:

What is the most common support material for a packed GC column?

  • ASilica gel
  • BFused silica
  • CAlumina
  • DDiatomaceous earth
  • EGlass

Q3:

Which of the following does reversed-phase chromatography refer to?

  • AA nonpolar stationary phase and a polar mobile phase
  • BA stationary phase and a mobile phase of similar polarities
  • CA polar stationary phase and a nonpolar mobile phase
  • DA polar stationary phase and a polar mobile phase
  • EA nonpolar stationary phase and a nonpolar mobile phase

Q4:

When separating a mixture by column chromatography, which of the following steps is not required?

  • AThe solvent mixture is selected to ensure good component separation.
  • BAll collected fractions are analyzed by thin-layer chromatography.
  • CAn eluent is passed through the column before adding the sample.
  • DThe solid phase is placed above a wool plug or glass frit.
  • EAn eluent is added to the top of the column to prevent drying.

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