Worksheet: The Beer–Lambert Law

In this worksheet, we will practice calculating concentrations from UV–vis spectra, using calibration curves or molar extinction coefficients.


Which of the following is a false statement about molar absorptivity coefficients?

  • AMolar absorptivities will be the same for each peak, regardless of wavelength, for a given molecule’s UV–vis spectrum.
  • BMolar absorptivity coefficients for organic molecules are typically 10,000 M−1⋅cm−1.
  • CMolar absorptivity coefficients are calculated based on absorbance and concentration.
  • DMolar absorptivities can be used to compare the ability to absorb light at a given wavelength of different chromophores.


The regression line from a plot of absorbance versus concentration yields 𝐴=2.31𝑐+0.002. If the absorbance of an unknown is measured to be 0.124, what is the concentration of the analyte?


Fill in the blank. The relationship between the analyte concentration and the intensity of measured radiation from thermal excitation methods, such as a flame or plasma, is .

  • Aparabolic
  • Blinear
  • Casymptotic
  • Dpolynomial
  • Eexponential


Fill in the blank: Background correction in flame atomic absorption spectroscopy can minimize the effect of .

  • Areactions between the analyte and matrix
  • Bscattering and absorption by the matrix of the analyte
  • Cnonvolatilization of the analyte
  • Dionization of the analyte

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