Worksheet: The Beer–Lambert Law

In this worksheet, we will practice calculating concentrations from UV–vis spectra, using calibration curves or molar extinction coefficients.


Which of the following is a false statement about molar absorptivity coefficients?

  • AMolar absorptivities will be the same for each peak, regardless of wavelength, for a given molecule’s UV–vis spectrum.
  • BMolar absorptivity coefficients for organic molecules are typically 10,000 M−1⋅cm−1.
  • CMolar absorptivity coefficients are calculated based on absorbance and concentration.
  • DMolar absorptivities can be used to compare the ability to absorb light at a given wavelength of different chromophores.

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