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Worksheet: Beer-Lambert Law

Q1:

Fill in the blank. The relationship between the analyte concentration and the intensity of measured radiation from thermal excitation methods, such as a flame or plasma, is .

  • Aparabolic
  • Bexponential
  • Cpolynomial
  • Dlinear
  • Easymptotic

Q2:

The regression line from a plot of absorbance versus concentration yields: 𝐴 = 2 . 3 1 𝑐 + 0 . 0 0 2 . If the absorbance of an unknown is measured to be 0.124, what is the concentration of the analyte?

Q3:

Fill in the blank. Background correction in flame atomic absorption spectroscopy can minimize the effect of .

  • AReactions between the analyte and matrix
  • BIonization of the analyte
  • CNon-volatilization of the analyte
  • DScattering and absorption by the matrix of the analyte

Q4:

Which of the following is a false statement about molar absorptivity coefficients?

  • AMolar absorptivity coefficients are calculated based on absorbance and concentration of the solution.
  • BMolar absorptivities can be used to compare the ability to absorb light at a given wavelength of different chromophores.
  • CMolar absorptivity coefficients for organic molecules are typically 1 0 0 0 0 M−1⋅cm−1.
  • DMolar absorptivities will be the same for each peak, regardless of wavelength, for a given molecule’s UV-Vis spectrum.