Worksheet: Culturing Microorgansims

In this worksheet we will practice describing the best methods to culture microorganisms, and using the process of binary fission to estimate population sizes of bacteria.

Q1:

A Streptococcus bacteria cell divides, on average, once every 30 minutes. How many bacteria cells will there be after 3.5 hours?

Q2:

A scientist timed how long it took a bacterial cell to replicate in hours. They would like to convert this to minutes. How can they convert a value from hours to minutes?

  • A 6 0
  • B + 6 0
  • C ÷ 6 0
  • D × 6 0

Q3:

A Staphylococcus bacteria cell divides, on average, once every 180 minutes.

How many bacteria cells will there be after 24 hours?

Q4:

When microorganisms are cultured, they are often grown on a culture medium (e.g., agar gel). Choose the correct words to complete the description of a culture medium:

A culture medium is a liquid or gel that contains appropriate to encourage the cells to .

  • Anutrients, die off
  • Btemperatures, replicate
  • Cnutrients, grow
  • Dorganisms, reproduce

Q5:

A Salmonella bacteria cell divides, on average, once every 45 minutes. How many bacteria cells will there be after 12 hours? Use the following formula: number of cells = 2numberofdivisions.

Q6:

What scientific term is given to the most common process by which bacteria cells replicate?

  • AMitosis
  • BBinary fission
  • CSynthesis
  • DMeiosis
  • EBinary fusion

Q7:

An E. coli bacteria cell divides, on average, once every 20 minutes. How many bacteria cells will there be after 3 hours?

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