Worksheet: Genetic Engineering

In this worksheet, we will practice describing the process of genetic engineering, and discussing its positive and negative implications.


Which of the following is not an example of a genetically engineered organism?

  • APotatoes that have had genes inserted into them to make them more resistant to pests
  • BMice that have been modified to develop human diseases like Alzheimer’s
  • CSoybeans that have had their DNA altered to produce more fatty acids
  • DObedient wolves that were bred together repeatedly to make docile dogs
  • EGolden rice that has been modified to contain more beta-carotene producing genes than normal rice


The soil bacterium Bacillus thuringiensis produces a protein called Bt toxin. Bt toxin is lethal to insects that consume it.

How can the Bt toxin be used in genetic engineering to benefit crop farming?

  • AThe Bt toxin can be incorporated into the genome of plants that are attacked by insects.
  • BThe Bt toxin can be added to fertilizers for plants that are attacked by insects.
  • CThe Bt toxin can be introduced into the genome of humans.
  • DThe Bt toxin can be introduced into the genome of insects.
  • EThe Bt toxin can be genetically engineered to make it less fatal to insects.


Genetic engineering involves manipulating the genome of an organism. What is a genome?

  • AAll of the DNA in an organism
  • BThe interaction between alleles in an organism
  • CAll the noncoding DNA in an organism
  • DThe physical characteristics of an organism
  • EAll the coding DNA in an organism


Which of the following best defines genetic engineering?

  • AGenetic engineering is the modification of the DNA of an organism.
  • BGenetic engineering is the repeated breeding of organisms with desired characteristics.
  • CGenetic engineering is the modification of the physical characteristics of an organism.
  • DGenetic engineering is a form of cloning.
  • EGenetic engineering is the creation of new animals.


The diagram provided shows a section of DNA being cut with a restriction enzyme.

What are the exposed bases on the pieces of DNA called?

  • ABlunt ends
  • BSticky ends
  • COpen ends
  • DGluey ends
  • ECut ends

Where do restriction enzymes cut DNA?

  • AWhere there is an ATGC all together
  • BWhere there is more than one hydrogen bond
  • CAt specific recognition sequences
  • DWhere any base repeats twice
  • EAt any point along the DNA strand

Genetic engineering involves combining multiple pieces of DNA. Why is it important that the pieces of DNA are cut with the same restriction enzymes?

  • ATo leave noncomplementary sticky ends
  • BTo have same-sized pieces of DNA
  • CTo save money
  • DTo leave complementary sticky ends
  • ETo leave complementary blunt ends


The diagram provided shows the basic process of genetic engineering.

What type of enzyme is the DNA and plasmid cut with?

  • AProtease
  • BDNA helicase
  • CRestriction enzymes
  • DDNA ligase
  • EDNA polymerase

What organism is the plasmid most commonly taken from in genetic engineering?

  • AVirus
  • BPlant
  • CAnimal
  • DBacteria
  • EFungi

What enzyme is used to join the section of DNA with the plasmid DNA?

  • ADNA ligase
  • BDNA helicase
  • CProtease
  • DDNA polymerase
  • ERestriction enzymes

Which of the following describes what happens after the section of DNA has been inserted into the plasmid?

  • AThe plasmid is taken and destroyed.
  • BThe plasmid is inserted into a human.
  • CThe plasmid is cloned.
  • DThe plasmid recombines with itself.
  • EThe plasmid is inserted back into a bacterial cell.

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