Worksheet: Mass Spectrometry Methods

In this worksheet, we will practice assessing the advantages and limitations of mass spectrometry methods and their suitability for particular samples.

Q1:

What are fast atom bombardment (FAB) and secondary ion mass spectrometry (SIMS) useful techniques for?

  • AProtein sequencing
  • BTrace metal analysis
  • CDepth profiling
  • DHalide analysis

Q2:

Why is inductively coupled plasma (ICP) typically only used as an ionization method for elemental analysis?

  • AIt requires the sample to be dissolved in a carrier gas prior to sample injection.
  • BAll structural information is lost due to extremely high plasma temperatures.
  • CICP produces an unpredictable fragmentation pattern.
  • DOnly low molecular weight molecules can be analyzed.

Q3:

Fill in the blank: The negative mode of chemical ionization is best suited for analysis of .

  • Acarbocations
  • Baromatics
  • Camines
  • Dhalides

Q4:

Which MS ionization technique is most useful for component analysis of metal-containing compounds, leaving fragments intact?

  • AElectron impact (EI)
  • BElectrospray ionization (ESI)
  • CInductively coupled plasma (ICP)
  • DMatrix assisted laser desorption ionization (MALDI)

Q5:

What are MALDI and ESI?

  • ASeparation methods used prior to MS analysis
  • BMass analysis techniques requiring external calibration
  • CIonization methods commonly used for biochemical analysis
  • DDetection methods used for spectral fingerprinting

Q6:

Which of the following is true about sector mass analyzers?

  • AThey are inexpensive and compact.
  • BThey are incompatible with ESI and MALDI ionization methods.
  • CThey can detect only low mass ranges.
  • DThey have low resolution and sensitivity.

Q7:

How do quadrupole and ion trap mass analyzers isolate ions for analysis?

  • ABy accelerating ions through an electric field
  • BBy accelerating ions through a magnetic field
  • CBy desorption from an electrode surface
  • DBy varying the applied voltage and radiofrequency potentials

Q8:

Which of the following is not a basic component of a mass spectrometer?

  • ADetector
  • BA sample inlet
  • CDiffraction grating
  • DMass analyzer
  • EIon source

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