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Lesson Worksheet: Oxidative Phosphorylation Biology

In this worksheet, we will practice describing the process of oxidative phosphorylation and recalling the products formed.


What molecule acts as the final electron acceptor in the electron transport chain?

  • AHydrogen
  • BWater
  • COxygen
  • DATP
  • ENAD


Which of the following best describes oxidative phosphorylation?

  • AThe formation of ATP via the phosphorylation of coenzymes NAD and FAD
  • BThe process by which electrons move down an electron transport chain, resulting in the formation of ATP from ADP
  • CThe process by which electrons are released from reduced coenzymes in the mitochondrial matrix
  • DThe active transport of hydrogen ions from the intermembrane space into the mitochondrial matrix


During oxidative phosphorylation, by what method of transport are hydrogen ions moved from the mitochondrial matrix to the intermembrane space?

  • AActive transport
  • BSimple diffusion
  • CFacilitated diffusion
  • DOsmosis


Hydrogen ions move from the intermembrane space to the mitochondrial matrix by facilitated diffusion through an enzyme called ATP synthase. What is produced when this happens?

  • AADP and oxygen
  • BATP and carbon dioxide
  • CATP
  • DADP
  • EPhosphorylated NAD


Why is oxidative phosphorylation considered an aerobic stage of respiration?

  • AIt can only happen in the presence of carbon dioxide.
  • BIt requires oxygen to be able to take place.
  • CIt produces oxygen as a by-product.
  • DEach stage of the reaction produces an oxidized molecule.


Where in a cell, specifically, is the electron transport chain associated with oxidative phosphorylation located?

  • AThe mitochondrial matrix
  • BThe nuclear envelope
  • CThe outer membrane of the mitochondria
  • DThe ribosomes of the mitochondria
  • EThe inner membrane of the mitochondria


Which of the following are products of oxidative phosphorylation?

  • ACarbon dioxide and ATP
  • BReduced NAD and FAD
  • CCarbon dioxide and water
  • DADP and oxygen
  • EATP and water


Which of the following statements about the reactions in oxidative phosphorylation is correct?

  • ACoenzymes FAD and NAD are reduced.
  • BCoenzymes NADH and FADH2 are oxidized.
  • CCoenzymes NAD and FAD are phosphorylated.


Assume oxidative phosphorylation is happening continuously in mitochondria. If the number of hydrogen ions in the intermembrane space significantly increases, what effect will this have?

  • AMore reduced NAD and FAD will be produced.
  • BFewer molecules of carbon dioxide will be released.
  • CMore ATP will be produced.
  • DLess ATP will be produced.


Which of the following best describes the role of chemiosmosis in oxidative phosphorylation?

  • AChemiosmosis is the movement of electrons down their electrochemical gradient, which generates energy in the electron transport chain.
  • BChemiosmosis is the chemical breakdown of ATP into ADP and inorganic phosphate.
  • CChemiosmosis is the movement of chemicals dissolved in water down their concentration gradient, across the mitochondrial membrane.
  • DChemiosmosis is the chemical breakdown of water into hydrogen and oxygen ions at the final stage of the electron transport chain.
  • EChemiosmosis is the movement of ions down their electrochemical gradient, which generates ATP.

This lesson includes 26 additional questions for subscribers.

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