Worksheet: Tissue Culture

In this worksheet, we will practice describing the processes of cloning in plants, and explaining the risks and benefits involved.

Q1:

Which of the following best describes the process of plant tissue culture?

  • ATissue culture takes small groups of cells from a plant and grows new, genetically unique plants.
  • BTissue culture takes a large cutting from a plant and grows genetically different new plants.
  • CTissue culture is a process that encourages sexual reproduction between plants.
  • DTissue culture takes small groups of cells from a plant and grows genetically identical new plants.
  • ETissue culture takes a large section of a plant and grows identical new plants from it.

Q2:

Plants can also be cloned by taking cuttings from a parent plant. How is taking cuttings different from tissue culture?

  • AGrowing cuttings requires scientific equipment and a laboratory.
  • BCuttings only use the flower of the parent plant.
  • CGrowing cuttings does not require human intervention.
  • DCuttings grow from larger pieces of the plant grown in soil.
  • ETissue culture is cheaper than taking cuttings.

Q3:

In the early stages of plant tissue culture, a callus is formed. What is a callus?

  • AA group of specialized plant cells
  • BA group of genetically different, undifferentiated plant cells
  • CA group of small plants
  • DA group of identical, undifferentiated plant cells

Q4:

Which of the following is a disadvantage of creating plants using tissue culture?

  • AIncreased allelic differences
  • BIncreased phenotypic variation
  • CInability to control growth conditions
  • DReduced genetic variation
  • EIncreased overcrowding in plants

Q5:

A florist wants to try to create some pink flowers from some red and white flowers he already has. Out of the methods listed below, which is the one he should use to do this?

  • ATaking cuttings
  • BAllowing them to reproduce sexually
  • CTissue culture

Q6:

A scientist wants to quickly produce many copies of an endangered orchid. Out of the methods listed below, which is the one she should use to do this?

  • ATaking cuttings
  • BTissue culture
  • CReproducing sexually with another plant

Q7:

The diagram given shows a basic outline of the three mains stages of plant tissue culture.

Which of the following best describes what is happening in the first stage?

  • AA small sample of cells is taken from the parent plant.
  • BThe cells are grown on an agar plate.
  • CThe roots are removed from the parent plant.
  • DA large cutting is taken from the parent plant.

Which of the following best describes what is happening in the second stage?

  • AThe small plants are grown in soil.
  • BA small sample of cells is taken from the parent plant.
  • CThe roots are grown on an agar plate.
  • DThe cells are grown on an agar plate.

Which of the following best describes what is produced in the final stage?

  • AA callus
  • BA group of genetically different plantlets
  • CA group of new cells
  • DA group of genetically identical plantlets

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