Worksheet: Thin-Layer Chromatography

In this worksheet, we will practice describing thin-layer chromatography methods, selecting mobile phases and methods for staining or detecting fractions.


When separating a solid mixture by thin-layer chromatography, which of the following steps is not required?

  • AThe base of the upright plate is immersed in an organic solvent.
  • BThe solid is dissolved in an organic solvent.
  • CA spot of solution is applied to the plate.
  • DMovement of the solvent front is accurately timed.
  • EMovement of the solute spots is accurately measured.

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