Worksheet: Selectivity and Resolution in Chromatography

In this worksheet, we will practice using the retention (Rf) values of fractions to calculate the resolution and selectivity of a chromatography method.


In a chromatographic analysis of an unknown mixture, compound A has a retention time of 5.56 minutes and a baseline width of 0.87, and compound B has a retention time of 6.32 minutes and a baseline width of 0.53 minutes. Are these peaks resolved, and what is the resolution between the two peaks?

  • ANo, 0.54
  • BYes, 0.54
  • CNo, 1.09
  • DYes, 1.09
  • EThere is not enough information given to determine resolution.

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