Worksheet: Selectivity and Resolution in Chromatography

In this worksheet, we will practice using the retention (Rf) values of fractions to calculate the resolution and selectivity of a chromatography method.

Q1:

In a chromatographic analysis of an unknown mixture, compound A has a retention time of 5.56 minutes and a baseline width of 0.87, and compound B has a retention time of 6.32 minutes and a baseline width of 0.53 minutes. Are these peaks resolved? What is the resolution between the two peaks?

  • AThere is not enough information given to determine resolution.
  • BYes, 1.09
  • CYes, 0.54
  • DNo, 1.09
  • ENo, 0.54

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