Worksheet: UV–Vis Sample Preparation

In this worksheet, we will practice describing UV–vis sample preparation, including filtration, dilution, cuvette selection, and reference selection.


Why must solutions with high concentrations be diluted prior to analysis via Beer’s law?

  • AThe photon source is too weak to provide accurate results.
  • BThere is no need to work with dilute concentrations; any concentration will work.
  • CThe relationship between absorbance and concentration is not linear at high concentrations.
  • DThe detector will reach its detection threshold.
  • EThe molar absorptivity of a compound is dependent on its concentration.


Which of the following would be the best solvent for a compound with a 𝜆max of 281 nm? You may assume that the compound is completely soluble in each.

  • AToluene (UV cutoff = 285 nm)
  • BBenzene (UV cutoff = 278 nm)
  • CAcetone (UV cutoff = 329 nm)
  • DEthanol (UV cutoff = 205 nm)


𝛽-carotene is a highly absorbing chromophore. Which solvent would be the best choice for UV–Vis analysis?

  • ABenzene
  • BHexyne
  • CGlycerine
  • DMethylene bromide
  • EEthanol


When preparing a calibration curve for sample analysis, which of the following is the best practice?

  • APreparing each sample “from scratch” to avoid cross contamination
  • BOnly using two data points to ensure a straight line
  • CUsing very concentrated solutions to ensure a good signal
  • DPreparing a stock solution and using serial dilution for the remaining standards


When a sample is analyzed by UV–vis spectroscopy, the results may be unreliable if the signal-to-noise ratio is too low. Which of the following is not a method for avoiding this issue?

  • ADissolving more of the analyte in the solvent
  • BUsing a cuvette with a shorter path length
  • CPreconcentrating the analyte solution
  • DUsing a solvent with a lower UV cutoff
  • EIncreasing the acquisition time for each absorbance measurement


A sample of 0.10 M triethylamine in hexane is prepared for analysis. Which material should be used as the blank reference?

  • AHexane
  • BTriethylamine
  • CAir
  • DWater
  • E0.10 M triethylamine in water

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