Lesson Worksheet: Glycolysis Biology

In this worksheet, we will practice describing the process of glycolysis and recalling the products made.


Why is glycolysis considered an anaerobic reaction?

  • ABecause it does not require oxygen
  • BBecause oxygen is not produced
  • CBecause oxygen is a key reactant
  • DBecause carbon dioxide is produced


In what part of a eukaryotic or prokaryotic cell does glycolysis take place?

  • ACytoplasm
  • BRibosomes
  • CCell membrane
  • DMitochondria
  • ENucleus


What is the net yield of ATP for one glucose molecule undergoing glycolysis?


How many molecules of pyruvate does one glucose molecule undergoing glycolysis produce?


How many molecules of reduced NAD are produced when one glucose molecule undergoes glycolysis?

  • A2 molecules
  • B4 molecules
  • C1 molecule
  • D6 molecules


Which compound is the primary reactant of glycolysis?

  • AGylceraldehyde 3-phosphate
  • BGlucose
  • CPyruvate
  • DOxygen
  • EReduced NAD


What 3-carbon compound is the final product of glycolysis?

  • APyruvate
  • BGlucose
  • CGlyceraldehyde 3-phosphate
  • D3-PGA
  • ENAD


Which of the following best describes the first major reaction that takes place in glycolysis?

  • AA glucose molecule is converted to reduced glucose by the addition of two hydrogen ions, catalyzed by the oxidation of reduced NAD.
  • BA phosphorylated fructose molecule is split into two 3-carbon molecules, known as glyceraldehyde 3-phosphate.
  • CA glucose molecule reacts with oxygen to form an unstable 6-carbon compound, which then breaks down into two molecules of glyceraldehyde 3-phosphate, catalyzed by the hydrolysis of ATP.
  • DA glucose molecule is converted to phosphorylated fructose by the addition of two phosphate molecules, catalyzed by the hydrolysis of ATP.


The diagram provided shows a basic outline of the process of glycolysis. The circles are used to represent the number of carbons in the compounds involved.

What compound has been replaced by the letter X?

  • AGlyceraldehyde 3-phosphate
  • BPyruvate
  • CGlucose
  • DSucrose

What compound has been replaced by the letter Y?

  • APhosphoglyceraldehyde
  • BPyruvate
  • CGlucose
  • DCarbon dioxide

What compound has been replaced by the letter Z?

  • AGlucose
  • BCarbon dioxide
  • CGlyceraldehyde 3-phosphate
  • DPyruvate


As phosphoglyceraldehyde is converted into pyruvate, what happens to the cofactor NAD?

  • AIt is phosphorylated.
  • BIt is oxidized.
  • CIt is reduced.
  • DIt is broken down into its component parts.

This lesson includes 22 additional questions for subscribers.

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