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Worksheet: Buffer pH Changes

Q1:

Acetate buffers are used in biochemical studies of enzymes and other chemical components of cells to prevent pH changes that might change the biochemical activity of these compounds.

An acetate buffer is made such that it is 0.10 M in acetic acid and 0.10 M in sodium acetate. By how much does the pH of the buffer change after 1.0 mL of 0 . 1 0 M N a O H is added to 100 mL of this buffer, giving a solution with a volume of 101 mL? 𝐾 a of C H C O H 3 2 is 1 . 8 Γ— 1 0   .

Q2:

Acetate buffers are used in studies of enzymes to prevent pH changes that might affect biochemical reactions. The 𝐾 a of acetic acid, the conjugate acid of acetate, is 1 . 8 Γ— 1 0   . Calculate to 2 decimal places the pH of an acetate buffer consisting of 0.10 M acetic acid and 0.10 M sodium acetate.

Q3:

A buffer solution is prepared from equal volumes of 0.200 M acetic acid and 0.600 M sodium acetate. The 𝐾 a of acetic acid is 1 . 8 0 Γ— 1 0 βˆ’ 5 . To 2 decimal places, what is the pH of the solution produced when 3.00 mL of 0 . 0 3 4 M H C l is added to 0.200 L of the buffer solution?