Worksheet: Aseptic Techniques

In this worksheet, we will practice explaining the importance of using aseptic techniques when culturing microorganisms.


What aseptic technique can be used to kill airborne pathogens surrounding a working area?

  • AWorking near open windows
  • BWorking near a bunsen flame
  • CWearing gloves
  • DSterilizing surfaces


Schools and universities often instruct students not to eat or drink in science labs.

Why is this instruction given?

  • AIt takes longer to clear up equipment if there is also food waste.
  • BIt is a distraction to be eating while working.
  • CIt is difficult to sterilize food and drink containers.
  • DFood and drink could become contaminated by microorganisms used in experiments.


The average body temperature of a human is 37C. When investigating bacterial growth, why should school laboratories incubate agar plates at 25C?

  • ATo ensure the growth of a large variety of microorganisms
  • BTo prevent the growth of harmful microorganisms that may infect humans
  • CTo prevent the growth of all microorganisms
  • DTo speed up the growth of their bacterial colonies


When scientists culture microorganisms in a lab, they should work aseptically. What is the aim of aseptic techniques?

  • ATo prevent the growth of any microorganisms
  • BTo provide the best conditions for microbial growth
  • CTo ensure all equipment is working properly
  • DTo prevent contamination from unwanted microorganisms


Which of the following is not an example of an aseptic technique?

  • AWearing gloves while working with microorganisms
  • BWorking close to an open window
  • CAutoclaving agar plates before and after use
  • DCleaning surfaces with disinfectant before use
  • EWorking close to a bunsen burner


Why should all doors and windows be closed when working aseptically?

  • ATo prevent humans from coming into contact with airborne microorganisms
  • BTo ensure optimum temperature for microorganism growth
  • CTo prevent the introduction of new microorganisms into the environment
  • DTo kill any airborne microorganisms in the working area


What process describes the unintended introduction of microorganisms that can impact a set of results in cell culture?

  • AInfection
  • BPathogenesis
  • CContamination
  • DCompromisation
  • EMicrobial growth


What part of an experimental setup describes a surface that provides nutrients for microorganisms to grow?

  • AWater
  • BAgar gel
  • CIncubator
  • DAutoclave
  • EPetri dish

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