Worksheet: Aseptic Techniques
In this worksheet, we will practice explaining the importance of using aseptic techniques when culturing microorganisms.
What aseptic technique can be used to kill airborne pathogens surrounding a working area?
- AWorking near open windows
- BWorking near a bunsen flame
- CWearing gloves
- DSterilizing surfaces
Schools and universities often instruct students not to eat or drink in science labs.
Why is this instruction given?
- AIt takes longer to clear up equipment if there is also food waste.
- BIt is a distraction to be eating while working.
- CIt is difficult to sterilize food and drink containers.
- DFood and drink could become contaminated by microorganisms used in experiments.
The average body temperature of a human is . When investigating bacterial growth, why should school laboratories incubate agar plates at ?
- ATo ensure the growth of a large variety of microorganisms
- BTo prevent the growth of harmful microorganisms that may infect humans
- CTo prevent the growth of all microorganisms
- DTo speed up the growth of their bacterial colonies
When scientists culture microorganisms in a lab, they should work aseptically. What is the aim of aseptic techniques?
- ATo prevent the growth of any microorganisms
- BTo provide the best conditions for microbial growth
- CTo ensure all equipment is working properly
- DTo prevent contamination from unwanted microorganisms
Which of the following is not an example of an aseptic technique?
- AWearing gloves while working with microorganisms
- BWorking close to an open window
- CAutoclaving agar plates before and after use
- DCleaning surfaces with disinfectant before use
- EWorking close to a bunsen burner
Why should all doors and windows be closed when working aseptically?
- ATo prevent humans from coming into contact with airborne microorganisms
- BTo ensure optimum temperature for microorganism growth
- CTo prevent the introduction of new microorganisms into the environment
- DTo kill any airborne microorganisms in the working area
What process describes the unintended introduction of microorganisms that can impact a set of results in cell culture?
- EMicrobial growth
What part of an experimental setup describes a surface that provides nutrients for microorganisms to grow?
- BAgar gel
- EPetri dish