Question Video: Predicting the Results of IAA Experiments Biology

The diagram provided shows a simple experiment used to investigate the effect of IAA on shoot growth. The shoots are set up as outlined in the diagram and then left for 7 days with a supply of water and nutrients. What would the expected result for shoot B be?

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Video Transcript

The diagram provided shows a simple experiment used to investigate the effect of IAA on shoot growth. The shoots are set up as outlined in the diagram and then left for seven days with a supply of water and nutrients. What would the expected result for shoot B be? (A) The shoot will grow toward the light. (B) The shoot will grow away from the light. (C) The shoot will stop growing. Or (D) the shoot will grow at a faster rate.

IAA belongs to a group of chemical messengers, or hormones, called auxins that play various roles in plants. IAA is produced mainly in the cells of the apical bud, which is the top bud of the plant. Some of the numerous functions of IAA are controlling growth by regulating cell division and elongation, sometimes in response to certain stimuli such as light.

Let’s take a closer look at the experiment shown in this diagram provided by the question. In shoot A, the cells in the apical bud should produce IAA as normal. IAA will diffuse from cell to cell down the plant shoot and accumulate in cells on the shaded side of the shoot. A high concentration of IAA in the cells on the shaded side of the shoot causes these cells to elongate comparatively more than the cells on the illuminated side of the shoot, resulting in directional growth of the shoot toward the light source.

In shoot B, the apical bud has been removed from the plant, which means that the cells that produce IAA have also been removed. This means that IAA cannot diffuse down the shoot to stimulate cell elongation in the cells below the apical bud. So this shoot is unlikely to grow any further.

In shoot C, the tip is removed and replaced with an agar block that contains IAA. This should result in directional growth of the shoot in a similar manner to what we observed in shoot A. IAA will diffuse down from the agar block to the cells below and accumulate on the shaded side of the shoot. This would stimulate them to elongate in the same asymmetrical manner as we saw in shoot A, causing shoot C to also bend in the direction of the light source.

Having explored the theory behind this experiment and its likely results, we can answer this question correctly. The expected result for shoot B is (C): the shoot will stop growing.

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