Question Video: Explaining the Steps of Melvin Calvin’s Photosynthetic Experiments | Nagwa Question Video: Explaining the Steps of Melvin Calvin’s Photosynthetic Experiments | Nagwa

Question Video: Explaining the Steps of Melvin Calvin’s Photosynthetic Experiments Biology

Melvin Calvin investigated photosynthesis in algae. The diagram demonstrates a simplified outline of his experiment. After the algae is subjected to a quick flash of light, why is it dropped into a flask of hot alcohol?

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Video Transcript

Melvin Calvin investigated photosynthesis in algae. The diagram demonstrates a simplified outline of his experiment. After the algae is subjected to a quick flash of light, why is it dropped into a flask of hot alcohol? (A) To begin digestion. (B) To initiate cell division. (C) To revive the cells. (D) To begin respiration. Or (E) to stop photosynthesis.

With his experiments, Melvin Calvin was able to make key advancements in our understanding of the process of photosynthesis. Calvin developed the experiment shown in the diagram.

In the first step of the experiment, algae was given access to carbon dioxide containing carbon-14. Carbon-14 is an isotope of carbon which has eight neutrons in its atomic nucleus instead of the six most carbon atoms have. In the second step, the algae were exposed to a brief flash of light and dropped in hot alcohol. This experiment allowed scientists to analyze the products of photosynthesis. We are trying to figure out why the algae is dropped into a flask of hot alcohol.

Hot alcohol kills the cells by a process called denaturation, which destroys the membranes and allows the contents of the cells to leak out.

Now that we know some background information, we can eliminate the incorrect answers. The alcohol will not initiate anything in the cells. It neither prompts digestion nor cell division nor respiration. So answers (A), (B), and (D) are all incorrect. Additionally, answer (C), to revive the cells, is also incorrect, because the algae is alive during the experiment and is killed upon contact with the hot alcohol.

As the cells die after dropping into the hot alcohol, photosynthesis stops. This allows the scientist to see what molecules are produced in the initial reaction to light, because the process of photosynthesis stopped soon after the brief light exposure. The correct answer is therefore (E). After the algae is subjected to a quick flash of light, it is dropped into a flask of hot alcohol to stop photosynthesis.

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