Lesson Plan: Factors Affecting Enzyme Action Biology

This lesson plan includes the objectives and prerequisites of the lesson teaching students how to describe the effect of temperature, pH, and substrate concentration on enzyme activity.


Students will be able to

  • describe and explain the effect of temperature, pH, enzyme concentration, and substrate concentration on enzyme activity,
  • define the terms optimum temperature and optimum pH,
  • identify the active site of an enzyme,
  • recall that enzymes will lower the activation energy of a reaction,
  • recall that enzymes and their active sites are made up of proteins and that if conditions change substantially, the structure of these proteins can be changed to denature the enzyme,
  • interpret graphical data to describe and explain changes in the rate of enzyme-controlled reactions.


Students should already be familiar with

  • the fact that enzymes are biological catalysts that speed up reactions,
  • the fact that enzymes have an active site into which a specific substrate fits,
  • the lock-and-key theory.

Nagwa uses cookies to ensure you get the best experience on our website. Learn more about our Privacy Policy.