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Lesson: UV–Vis Sample Preparation Chemistry

In this lesson, we will learn how to describe UV–vis sample preparation, including filtration, dilution, cuvette selection, and reference selection.

Lesson Worksheet

Q1:

Which of the following would be the best solvent for a compound with a of 281 nm? You may assume that the compound is completely soluble in each.

Q2:

-carotene is a highly absorbing chromophore. Which solvent would be the best choice for UV–Vis analysis?

Q3:

When preparing a calibration curve for sample analysis, which of the following is the best practice?