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Lesson: Column Chromatography

In this lesson, we will learn how to describe purification by column chromatography, selecting mobile and stationary phases and troubleshooting errors.

Worksheet • 4 Questions

Q1:

What is the most common support material for a packed GC column?

  • ADiatomaceous earth
  • BGlass
  • CSilica gel
  • DFused silica
  • EAlumina

Q2:

Which of the following statements about columns containing more theoretical plates is true?

  • AColumns containing more theoretical plates are better suited to separate a complex mixture.
  • BColumns containing more theoretical plates lend themselves to component mixing.
  • CColumns containing more theoretical plates make separations imposssible.
  • DColumns containing more theoretical plates interact irreversibly with the analyte.
  • EColumns containing more theoretical plates take a long time to perform separations.

Q3:

Reversed-phase chromatography refers to which of the following?

  • AA nonpolar stationary phase and a polar mobile phase
  • BA polar stationary phase and a polar mobile phase
  • CA stationary phase and mobile phase of similar polarities
  • DA polar stationary phase and a nonpolar mobile phase
  • EA nonpolar stationary phase and a nonpolar mobile phase

Q4:

When separating a mixture by column chromatography, which of the following steps is not required?

  • AAll collected fractions are analysed by thin-layer chromatography.
  • BEluent is passed through the column before adding the sample.
  • CThe solvent mixture is selected to ensure good component separation.
  • DEluent is added to the top of the column to prevent drying.
  • EThe solid phase is placed above a wool plug or glass frit.
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