When separating a solid mixture by thin-layer chromatography, which of the following steps is not required?
A reaction mixture is analyzed by thin-layer chromatography before (A) and after (B) a reaction takes place, using a silica TLC plate and a mixture of hexane and ethyl acetate. In the diagram showing the TLC plate, the positions of the separated fractions are marked as spots between the baseline (solid line) and solvent front (dashed line).
Which spot corresponds to the product of the reaction with the highest polarity?
Which spot corresponds to the limiting reactant in this reaction?
Estimate the value of fraction 5, to 1 significant figure.
The stationary phase in ion exchange chromatography (IEC) is a cross-linked polymer resin with covalently attached functional groups. Which of the following is not a typical functional group used for IEC?