Lesson Flashcards: Using Restriction Enzymes

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Definition

5′ and 3′

The numbers that refer to the specific carbon in the deoxyribose backbone of DNA to which a new deoxyribonucleotide bonds

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Definition

EcoRI

The restriction enzyme that recognizes the DNA sequence GAATTC

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Definition

DNA (deoxyribonucleic acid)

A nucleic acid molecule that carries the genetic instructions in living things and typically takes the form of a double helix

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Definition

Enzyme

A biological catalyst that speeds up the rate of reactions without being used up

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Definition

Blunt ends

The DNA sequences that are produced by the cutting of restriction enzymes that have no unpaired sequences and terminate with a base pair

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Definition

Palindrome

A word or sequence that reads the same forward and backward

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Definition

Hydrolysis

A chemical reaction that breaks a bond between molecules through the addition of a water molecule

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Definition

Restriction enzyme (restriction endonuclease)

An enzyme that cuts DNA at a specific, typically palindromic, sequence

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Definition

Recognition sequence

A specific sequence of DNA that a restriction enzyme recognizes and cleaves

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Definition

Recombinant DNA

DNA combined from at least two different sources to form new genetic information not previously found in the genome

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