5′ and 3′
Lesson Flashcards: Using Restriction Enzymes
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The numbers that refer to the specific carbon in the deoxyribose backbone of DNA to which a new deoxyribonucleotide bonds
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EcoRI
The restriction enzyme that recognizes the DNA sequence GAATTC
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DNA (deoxyribonucleic acid)
A nucleic acid molecule that carries the genetic instructions in living things and typically takes the form of a double helix
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Enzyme
A biological catalyst that speeds up the rate of reactions without being used up
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Blunt ends
The DNA sequences that are produced by the cutting of restriction enzymes that have no unpaired sequences and terminate with a base pair
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Palindrome
A word or sequence that reads the same forward and backward
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Hydrolysis
A chemical reaction that breaks a bond between molecules through the addition of a water molecule
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Restriction enzyme (restriction endonuclease)
An enzyme that cuts DNA at a specific, typically palindromic, sequence
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Recognition sequence
A specific sequence of DNA that a restriction enzyme recognizes and cleaves
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Recombinant DNA
DNA combined from at least two different sources to form new genetic information not previously found in the genome
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Score